6/14/2023 0 Comments Dynamic light scattering method![]() At high concentrations, particle-particle interactions and multiple light scattering can become dominant and can result in apparent particle sizes that differ between concentrated and dilute suspensions. Dynamic light scattering (DLS), also known as photon correlation spectroscopy (PCS), is a very powerful tool for studying the diffusion behaviour of macromolecules in solution. DLS is an effective tool to characterize dynamic parameters of nanoparticles including the diffusion coefficient and particle size within a colloidal system. However, specific requirements for the instrument setup and specification of test sample preparation are required for concentrated suspensions. The principle of dynamic light scattering for a concentrated suspension is the same as for a dilute suspension. ISO 22412:2017 is applicable to the measurement of a broad range of dilute and concentrated suspensions. ![]() ![]() ![]() DLS is also referred to as "quasi-elastic light scattering (QELS)" and "photon correlation spectroscopy (PCS)," although PCS actually is one of the measurement techniques. In dynamic light scattering (DLS), the time-dependent fluctuations in the scattered light are measured by a single photon counting module. ISO 22412:2017 specifies the application of dynamic light scattering (DLS) to the measurement of average hydrodynamic particle size and the measurement of the size distribution of mainly submicrometre-sized particles, emulsions or fine bubbles dispersed in liquids. ![]()
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